Everything about Pipes totally explained
» This page is about the biochemical buffer PIPES. For information about other pipes, see Pipe.
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PIPES is the common name for
piperazine-N,N′-bis(2-ethanesulfonic acid), a frequently used
buffering agent in
biochemistry. It is an ethanesulfonic acid buffer developed by Good et al in the 1960's.
Applications
PIPES has
pKa near the physiological
pH which makes it useful in
cell culture work. PIPES has been documented minimizing
lipid loss when buffering glutaraldehyde
histology in plant and animal
tissues. Fungal zoospore fixation for
fluorescence microscopy and
electron microscopy were optimized with a combination of
glutaraldehyde and
formaldehyde in PIPES buffer. It has a negligible capacity to bind
divalent ions.
Further Information
Get more info on 'Pipes'.
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